Suspected Rabies in Humans and Animals, Laikipia County, Kenya

flab gene was targeted by using multiplex qPCR according to a previous described protocol (1). For quality control of qPCRs, we included positive and negative controls. Sequences of qPCR products were analyzed and compared with sequences available in GenBank. Overall preva-lences were 1.50% for B. miyamotoi, 3.42% for A. phago-cytophilum, and 5.34% for Candidatus N. mikurensis. Prevalences of each pathogen in specific varied by locality (Table). No co-infections were detected. We analyzed flab, msp2, and groEL gene sequences obtained by qPCR. These sequences showed 99%–100% identities with gene sequences of B. miyamotoi (GenBank accession no. KJ847050), A. phagocytophilum (accession no. KP164415), and Candidatus N. mikurensis (accession no. FJ966365). In Romania, the density of Ix. ricinus ticks is high and their host diversity is extensive (7). However, data for effects of tickborne pathogens on public health are scarce in this country. In this study, we detected B. miyamotoi, A. phagocytophilum, and Candidatus N. mikurensis in questing Ix. ricinus ticks in Romania, which confirms the emerging trend of these pathogens in Europe. Because of the scarcity of information on human infections with these pathogens in Romania, serologic and molecular investigations and their implementation are needed for diagnosis, which might help in assessing the effect of these pathogens on public health. prevalence of Borrelia burgdorferi genospecies in questing Ixodes ricinus from Romania: a countrywide study. Chitimia L. Co-infection with 'Candidatus Neoehrlichia mikurensis' and Borrelia afzelii in an Ixodes ricinus tick that has bitten a human in Romania. To the Editor: Dog bites are a serious public health problem because of the associated risk for rabies virus exposure in countries to which the virus is endemic (1,2).